| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dyjak, Ella S (16)
|
CTCAC |
53.93 |
55.42 |
1.49 |
| 2 |
Dunnigan, Megan L (15)
|
CTUN |
53.64 |
56.31 |
2.67 |
| 3 |
D'Arcy, Alessandra J (15)
|
CTCAC |
57.63 |
57.58 |
-0.05 |
| 4 |
Benavides, Hannah L (16)
|
CTCAC |
57.30 |
58.11 |
0.81 |
| 5 |
Prenoveau, Kenyon D (17)
|
CTFVYT |
57.64 |
58.54 |
0.90 |
| 6 |
Hayes, Karrah M (16)
|
CTUN |
55.94 |
59.83 |
3.89 |
| 7 |
Agrafojo, Elisabeth A (15)
|
CTUN |
1:01.28 |
1:02.72 |
1.44 |
| 8 |
Sciarra, Paige K (15)
|
CTUN |
1:01.70 |
1:03.58 |
1.88 |
| 9 |
Yanicky, Emma R (15)
|
CTUN |
1:05.64 |
1:03.62 |
-2.02 |
| 10 |
Dziatko, Sarah M (17)
|
CTLEHY |
1:06.00 |
1:03.66 |
-2.34 |